Portable Real-Time PCR System - ToronPCR™ P Series
Portable 16-Well Real-Time PCR System (qPCR) with 2 or 4 Fluorescence Channels
The portable real-time PCR system ToronPCR™ P Series brings 16-well qPCR to field sites, quarantine checkpoints, and small laboratories without a PC or a bench-sized footprint. It monitors fluorescence during every amplification cycle, so operators read quantitative results while the run progresses. The series offers a 2-channel model and a 4-channel model. Both weigh 5.9 kg and measure 200 × 297 × 148 mm, which makes the instrument easy to carry between sampling locations.
A 7-inch Windows touchscreen handles run setup, data collection, analysis, and storage on the instrument itself. The software interprets positive and negative results automatically, with no separate calculations. Analysis modes include qualitative and quantitative detection, melting curve, genotyping, and isothermal amplification. The open software accepts reagent kits from different manufacturers, so laboratories keep their existing assays.
A semiconductor chilling plate drives the thermal block at up to 6 °C/s, and a maintenance-free LED source with a high-sensitivity photodetector reads fluorescence from 0.2 mL tubes. The dynamic range spans 1 to 10¹⁰ copies with a linearity of |r| ≥ 0.999. USB and network ports move data to a PC or straight to a printer. Food safety, veterinary, agricultural, and public health teams use this qPCR instrument for on-site pathogen screening.
Portable Real-Time PCR System - ToronPCR™ P Series Applications
The ToronPCR™ P Series moves quantitative PCR out of the central laboratory and into the places where samples are collected.
- On-Site Pathogen Detection: screens samples for bacteria and viruses at the point of collection, which shortens the wait for a laboratory result
- Food Safety Testing: detects foodborne pathogens such as Salmonella and E. coli in raw materials, finished products, and irrigation water
- Inspection and Quarantine: supports rapid screening at ports of entry and border checkpoints, where results guide release or hold decisions
- Disease Prevention and Control: tracks outbreak targets in public health programs with qualitative or quantitative assays
- Agriculture and Forestry: identifies plant pathogens in soil and tissue samples during field surveys, before planting or treatment decisions
- Veterinary and Livestock Health: tests animal samples for viral and bacterial diseases at the farm instead of a distant laboratory
- Biochemistry and Molecular Biology Research: measures gene copy number, runs melting curves, and performs genotyping, with four-target multiplexing on the 4-channel model
Portable Real-Time PCR System - ToronPCR™ P Series Key Features
The ToronPCR™ P Series pairs field-ready hardware with a complete onboard analysis workflow.
The Instrument Can Be Set to 4 Channels and A Variety of Test Item Types
Diversified Program Settings
- Compact, Carry-Ready Body: weighs 5.9 kg in a 200 × 297 × 148 mm footprint, so one person moves it between sites
- Stand-Alone 7-Inch Touchscreen: handles setup, testing, result judgment, and data saving on the instrument without a connected PC
- 2 or 4 Fluorescence Channels: matches the instrument to the assay, from single-dye detection to multiplex panels with FAM/SYBR Green I, HEX/JOE/VIC, ROX, and Cy5
- 16-Well Throughput: runs up to 16 reactions per cycle in 0.2 mL 8-strip or single tubes, with 15 to 100 μL sample volumes
Function Layout Is Simple and Easy to Under-stand
Easy and Fast Data Export
- Automatic Result Interpretation: classifies samples as positive or negative without manual calculation, which speeds screening
- Multiple Analysis Modes: covers qualitative and quantitative detection, melting curve, genotyping, and isothermal amplification in one instrument
- Maintenance-Free LED Light Source: delivers bright, stable excitation with no lamp replacement
- High-Sensitivity Photodetector: keeps readings stable from run to run
- Fast Thermal Cycling: reaches heating and cooling rates up to 6 °C/s with ±0.2 °C block uniformity at 95 °C
- Open Reagent Compatibility: works with kits from different manufacturers, so laboratories keep their preferred chemistries
- Flexible Program Setup: accepts protocols laid out on the touchscreen, imported from a USB drive, or recalled from a project library
- Fast Data Export and Printing: sends results through USB or the network port as PDF or Excel files, or directly to a printer
- Built-In Protection: raises an alarm and protects the instrument during short circuit, open circuit, and over-temperature events
Theory and Method
Real-time PCR copies a target sequence through repeated thermal cycles: heat separates the DNA strands, primers anneal at a lower temperature, and a polymerase extends them. Each cycle roughly doubles the target. A fluorescent reporter tracks this growth. Intercalating dyes such as SYBR Green I fluoresce when they bind double-stranded DNA, while hydrolysis probes release a fluorophore from its quencher during extension. Signal intensity rises in proportion to the amplified product.
The instrument reads fluorescence after every cycle and plots it against cycle number. The threshold cycle (Ct) is the cycle where the signal rises above background. A sample with more starting template reaches the threshold sooner, so Ct falls as template concentration rises. A standard curve of known concentrations converts Ct into copy number; a slope near -3.33 marks roughly 100% efficiency. Melting curve analysis then heats the product and tracks fluorescence loss, which reveals whether a single specific amplicon formed.
The ToronPCR™ P Series applies this principle with a semiconductor chilling plate that cycles the 16-well block at up to 6 °C/s. A maintenance-free LED excites the dyes, and a photodetector reads emission through separate channels. The 2-channel model covers 470 to 510 nm and 530 to 565 nm (FAM, SYBR Green I, HEX, JOE, VIC). The 4-channel model adds 585 to 620 nm and 630 to 665 nm (ROX, Cy5) for four-target multiplexing.
Portable Real-Time PCR System - ToronPCR™ P Series Technical Specifications
| Parameter | ToronPCR™ P2 (2-Channel Model) | ToronPCR™ P4 (4-Channel Model) |
|---|---|---|
| Detection Throughput | 16-well | 16-well |
| Sample Volume | 15 to 100 μL | 15 to 100 μL |
| Consumable | 0.2 mL thin-wall transparent 8-strip tube; 0.2 mL thin-wall transparent single tube | 0.2 mL thin-wall transparent 8-strip tube; 0.2 mL thin-wall transparent single tube |
| Dynamic Range | 1 to 10¹⁰ copies | 1 to 10¹⁰ copies |
| Linearity | |r| ≥ 0.999 | |r| ≥ 0.999 |
| Number of Channels | 2 | 4 |
| Channel Wavelength Range | Channel 1: 470 to 510 nm; Channel 2: 530 to 565 nm | Channel 1: 470 to 510 nm; Channel 2: 530 to 565 nm; Channel 3: 585 to 620 nm; Channel 4: 630 to 665 nm |
| Channel Dyes | F1: FAM, SYBR Green I, etc.; F2: HEX, JOE, VIC, etc. | F1: FAM, SYBR Green I, etc.; F2: HEX, JOE, VIC, etc.; F3: ROX, etc.; F4: Cy5, etc. |
| Excitation Light Source | LED (maintenance-free) | LED (maintenance-free) |
| Detection Element | Photodetector (PD) | Photodetector (PD) |
| Temperature Control Mode | Block / tube | Block / tube |
| Temperature Range | 4 to 99.9 °C | 4 to 99.9 °C |
| Temperature Control Method | Semiconductor chilling plate | Semiconductor chilling plate |
| Temperature Accuracy | ±0.15 °C | ±0.15 °C |
| Temperature Control Precision | 0.1 °C | 0.1 °C |
| Temperature Uniformity | ±0.2 °C at 95 °C | ±0.2 °C at 95 °C |
| Maximum Heating Rate | 6 °C/s | 6 °C/s |
| Maximum Cooling Rate | 6 °C/s | 6 °C/s |
| Average Heating Rate | 4 °C/s | 4 °C/s |
| Average Cooling Rate | 4 °C/s | 4 °C/s |
| Melting Temperature Resolution | Minimum difference 0.1 °C | Minimum difference 0.1 °C |
| Heated Lid Temperature | 30 to 110 °C | 30 to 110 °C |
| Display | 7-inch touchscreen (Windows) | 7-inch touchscreen (Windows) |
| Data Interfaces | USB, network port, printer connection | USB, network port, printer connection |
| Data Export Format | PDF, Excel | PDF, Excel |
| Input Power | 100 to 240 V AC, 50 to 60 Hz | 100 to 240 V AC, 50 to 60 Hz |
| Maximum Power | 200 W | 200 W |
| Safety Protection and Alarm | Short circuit, open circuit, and over-temperature protection with alarm | Short circuit, open circuit, and over-temperature protection with alarm |
| Dimensions (W × D × H) | 200 × 297 × 148 mm | 200 × 297 × 148 mm |
| Net Weight | 5.9 kg | 5.9 kg |