Large-Volume Nucleic Acid Extractor - ToronNAE™ LV Series
ToronNAE™ LV15 and LV20 Large-Volume Magnetic Bead Nucleic Acid Purification System
The ToronNAE™ LV Series large-volume nucleic acid extractor handles sample inputs of 1 mL up to 20 mL and elutes purified DNA and RNA into as little as 20 µL. Oncology labs, liquid biopsy programs, and pathology departments use it to concentrate scarce cell-free DNA from plasma and recover DNA directly from FFPE tissue blocks. Each run processes 1 to 24 samples in strip-format cartridges. The design pairs magnetic rods for large sample wells with a pipetting head for small-volume steps.
Two models cover different volume needs. The ToronNAE™ LV15 accepts 2 mL, 8 mL, and 15 mL cartridges and runs full FFPE block extraction, including dewaxing inside the instrument. The ToronNAE™ LV20 uses a dedicated 20 mL cartridge for the largest plasma, body fluid, and concentrated environmental samples. Both models share the same pipettor, heating system, and software. Open software works with most magnetic bead extraction reagents on the market.
Eluates collect in separate elution tubes that operators remove and store with no further transfer. Bilateral sensors on the moving components detect uneven or misplaced consumables and stop the run before a crash. A UV lamp, HEPA filtration, and exhaust fans contain potential contaminants. The Windows-based software runs on a 10-inch touchscreen with user authorization levels. An external barcode scanner records sample and reagent information.
Large-Volume Nucleic Acid Extractor - ToronNAE™ LV Series Applications
The ToronNAE™ LV Series serves labs where target nucleic acid is dilute and sample volume is large, or where tissue arrives fixed in paraffin. Its small elution volume concentrates the recovered material for sensitive downstream assays.
- Liquid Biopsy and cfDNA Analysis: cell-free DNA extraction using 1 mL to 20 mL of plasma for tumor mutation detection by qPCR, digital PCR, or NGS
- FFPE Tissue Pathology: automated dewaxing, lysis, and DNA extraction directly from paraffin-embedded tissue blocks (ToronNAE™ LV15)
- Oncology Research: high-purity DNA from tumor tissue and plasma for biomarker and companion diagnostic studies
- Prenatal and Noninvasive Testing Research: cell-free DNA recovery from maternal plasma samples
- Viral and Pathogen Detection: large-input extraction of whole blood, sputum, and body fluids that raises sensitivity for low-titer pathogens
- Wastewater and Environmental Surveillance: nucleic acid extraction from concentrated wastewater, water, and environmental samples for pathogen monitoring
- Stool and Microbiome Studies: DNA extraction from stool for gut microbiome and gene library work
- Animal and Plant Research: DNA extraction from blood and animal or plant tissues
Large-Volume Nucleic Acid Extractor - ToronNAE™ LV Series Key Features
- Nucleic acid extraction area
Suitable for various types of cartridges, mainly used for loading extraction reagents, can be pre-packaged, and the instrument is equipped with puncture structure
- Sample consumable rack
Used for placing various consumables during the extraction process, such as tips, product recovery tube, etc
The ToronNAE™ LV Series combines large-volume capture, small-volume elution, and FFPE automation in one 24-sample platform. Each feature targets higher yield from dilute samples, less manual handling, and fewer run errors.
- Large-Volume Extraction, Small-Volume Elution: processes up to 15 mL (LV15) or 20 mL (LV20) per sample and elutes into 20 µL to 200 µL
- 1 to 24 Samples per Run: strip-format cartridges let labs run partial batches without wasting consumables
- Segmented Mixing and Magnetic Capture: tip hovering and segmented rod absorption keep beads suspended and recovered across the full sample volume
- Side Magnetic Absorption for Small Volumes: captures beads on the tip wall during aspiration for efficient low-volume washes and elution
- Automated FFPE Dewaxing (LV15): dewaxing, layering, and lower-phase transfer run inside the instrument without a separate dry bath
- Pre-Packaged Reagent Support: the extraction area accepts pre-filled cartridges with a puncture structure that pierces the sealing film automatically
- Direct-to-Storage Elution Tubes: eluates collect in separate tubes ready for storage, with no extra transfer step
- Precision Pipetting Head: 20 µL to 1000 µL range with CV of 1% or less at 500 µL and 1000 µL
- Consumable Anti-Collision Detection: bilateral sensors flag misplaced consumables during self-check and runs, preventing failed extractions and machine damage
- Contamination Control: UV lamp, HEPA filtration, exhaust fans, and disposable consumables limit cross-contamination
- Open, Intuitive Software: visual interface on a 10-inch touchscreen, compatible with most magnetic bead extraction reagents
- User Authorization Management: administrator-level control over programs and instrument settings
- Barcode Scanner Support: external scanner input records sample and reagent information
- Optional Visual System: automatically confirms consumable types and positions before the run
Theory and Method
Magnetic bead extraction isolates nucleic acids on the surface of silica-coated paramagnetic particles. A lysis buffer with chaotropic salts breaks open cells and strips proteins off DNA and RNA, which then bind tightly to the beads. Wash buffers remove salts, proteins, and inhibitors, and a low-salt elution buffer releases the purified nucleic acid. Large inputs matter for targets such as cfDNA, which circulates at very low concentrations in plasma. More starting volume means more target copies in the final eluate.
Large volumes make bead capture harder because beads disperse widely. The ToronNAE™ LV Series handles the sample well with segmented mixing: the tip hovers at several depths and moves side to side while it dispenses and aspirates. Magnetic rods then sweep the well in segments to collect beads from the full liquid column. For wash and elution wells, an external magnet pulls beads against the inner tip wall during aspiration. This side capture recovers beads from volumes as small as 20 µL.
FFPE extraction adds a dewaxing step. The ToronNAE™ LV15 heats the block in a capped well with a xylene-free dewaxing and lysis buffer, which melts the paraffin and digests the tissue. The paraffin layer separates on top, and the instrument transfers the lower aqueous phase automatically. Pre-filled FFPE cartridges carry a pierceable film, so the pipettor punctures the seal instead of requiring manual peeling. Heated lysis and elution wells reach 120 °C with ±1 °C accuracy at 100 °C.
Large-Volume Nucleic Acid Extractor - ToronNAE™ LV Series Technical Specifications
Model-Specific Specifications
| Parameter | ToronNAE™ LV15 | ToronNAE™ LV20 |
|---|---|---|
| Throughput | 1 to 24 samples | 1 to 24 samples |
| Sample Processing Volume | 1 mL to 15 mL | 1 mL to 20 mL |
| Compatible Cartridges | 2 mL, 8 mL, 15 mL | 20 mL only |
| FFPE Block Extraction | Yes (with automated dewaxing) | No |
| Rated Power | 600 W | 600 W |
Shared Specifications (Both Models)
| Parameter | Specification |
|---|---|
| Working Principle | Magnetic bead method: magnetic rod type plus pipetting type with tip dispensing and aspirating mixing |
| Sample Types | FFPE block (LV15), whole blood, plasma, stool, sputum, animal and plant tissue |
| Elution Volume | 20 µL to 200 µL (minimum 20 µL for FFPE block or 1 mL cfDNA) |
| Stability | CV ≤ 5% |
| Pre-Packaged Reagents | Supported; automatic film piercing (2 mL cartridge) |
| Pipetting Range | 20 µL to 1000 µL |
| Pipetting Precision (CV) | 20 µL: ≤ 5%; 100 µL: ≤ 1.5%; 500 µL: ≤ 1%; 1000 µL: ≤ 1% |
| Pipetting Accuracy | 20 µL: ±8%; 100 µL: ±2%; 500 µL: ±1%; 1000 µL: ±1% |
| Heating Purpose | Incubation during lysis and elution |
| Temperature Control Range | Room temperature to 120 °C |
| Average Heating Rate | ≥ 0.2 °C/s (at 25 °C ambient) |
| Temperature Accuracy | ≤ ±1 °C at 100 °C (25 °C ambient) |
| Temperature Uniformity | ≤ 3 °C at 100 °C (25 °C ambient) |
| Temperature Stability | ≤ ±0.5 °C at 100 °C (25 °C ambient) |
| Software | Windows-based, 10-inch touchscreen |
| Account Management | User authorization levels; customizable programs and instrument settings |
| Instrument Ports | USB, LAN |
| Information Management | External barcode scanner support |
| Visual System | Optional (confirms consumable type and position) |
| Safety Functions | Bilateral anti-collision sensors with alarm |
| Pollution Control | Built-in HEPA filtration, UV sterilization, exhaust fans |
| Votlage | AC 220 V, 50 Hz (110V is also available) |
| Dimensions (W × D × H) | 740 × 640 × 586 mm |
| Weight | 85 kg |
Deck Layout
| Area | Description |
|---|---|
| Nucleic Acid Extraction Area | Holds cartridges of each size for pre-filled extraction reagents, with a film puncture structure |
| Sample Consumable Area | Holds tips, sample tubes, and elution recovery tubes |
Consumables and Accessories
| Item | Description |
|---|---|
| 2 mL Cartridge | Strip cartridge for FFPE and standard extractions (LV15) |
| 8 mL Cartridge | Strip cartridge for mid-volume cfDNA extraction (LV15) |
| 15 mL Cartridge | Strip cartridge for large-volume cfDNA extraction (LV15) |
| 20 mL Cartridge | Strip cartridge for maximum-volume extraction (LV20) |
| Cap | Seals wells to prevent evaporation during FFPE dewaxing |
| 1 mL Special Tip and Tip Holder | Tips for pipetting, mixing, and magnetic absorption |
| Magnetic Rod Comb and Comb Holder | Rod covers for residue-free bead capture in large sample wells |
| 5 mL and 12 mL Sample Tubes | Tubes for loading samples |
| 1.5 mL and 2 mL Storage Tubes | Elution tubes for direct storage of purified nucleic acid |
| 2 mL Cartridge Holder | Metal or plastic holder for 2 mL cartridges |
| 8/15/20 mL Cartridge Holder | Metal or plastic holder for 8 mL, 15 mL, and 20 mL cartridges |