High-Throughput Nucleic Acid Extractor - ToronNAE™ Series
ToronNAE™ Series Magnetic Bead Nucleic Acid Purification System
The ToronNAE™ Series high-throughput nucleic acid extractor purifies DNA and RNA through magnetic bead separation in 30 to 60 minutes per run. Three models share one platform: the ToronNAE™ 96 processes up to 96 samples at 1 mL, the ToronNAE™ 48 handles 48 samples at 3 mL, and the ToronNAE™ 24 runs 24 samples at 10 mL. Labs pick the model that matches their sample volume and batch size. Each unit works with most commercial magnetic bead extraction kits.
A center rotary table moves eight plate positions under a fixed magnetic head, which keeps the footprint compact and the motion stable. Two of those positions heat for lysis and elution, and labs can request up to four heated positions shaped to their reagent kit. The 7-inch color touchscreen lets operators edit parameters and programs without a computer. Eight preset programs come loaded, and the system stores up to 100 user programs. Each run ends with an auto-generated report for traceability.
Contamination control runs through the whole design. Disposable tip combs and plates isolate every sample, while a drip shield and software-controlled bead drying keep reagents from splashing between wells. UV sterilization, fan exhaust, and built-in LED lighting support clean, visible operation. The system self-checks at startup, detects missing kits, and protects runs during power loss. Optional integration with robotic arms and liquid handlers lets the extractor join a fully automated workflow.
High-Throughput Nucleic Acid Extractor - ToronNAE™ Series Applications
The ToronNAE™ Series serves labs that extract nucleic acids in volume, including routine swab testing and large-input cell-free DNA work. Its three reaction volumes let one platform cover both high-throughput and high-volume needs.
- Infectious Disease Testing: batch DNA and RNA extraction from swabs, blood, serum, and body fluids ahead of real-time PCR
- Third-Party and Reference Laboratories: full 96-sample runs for high daily testing volumes with traceable run reports
- Cell-Free DNA and Liquid Biopsy: large-volume extraction of cfDNA from plasma using the 10 mL reaction system
- Microbiome and Metagenomics: DNA extraction from stool and intestinal samples for microbial gene library preparation
- Genetic Disease Screening and Oncology Research: high-purity DNA extraction for genotyping and tumor-related studies
- Animal Health and Husbandry: pathogen screening and genotyping from animal blood, tissue, and swab samples
- Plant and Germplasm Research: DNA extraction from plant tissue and seed for breeding and genetic resource studies
- Food Safety and Environmental Testing: nucleic acid purification for foodborne pathogen, residue, and environmental microbiology assays
- Forensic Laboratories: consistent DNA extraction for identification and casework samples
High-Throughput Nucleic Acid Extractor - ToronNAE™ Series Key Features
The ToronNAE™ Series combines high-throughput capacity, open reagent compatibility, and layered contamination control on a compact rotary platform. Each feature targets faster batches, consistent yields, and traceable results.
- Three Throughput and Volume Options: 96 × 1 mL, 48 × 3 mL, or 24 × 10 mL per run, so one platform covers routine swabs and large-volume cfDNA samples
- Fast 30 to 60 Minute Extraction: complete DNA or RNA purification for a full plate in under an hour, depending on the kit
- Center Rotary Table Design: rotates eight plate positions under the magnetic head for a small footprint and stable motion
- Customizable Heated Positions: two heated positions as standard, expandable to four, with heater shapes adapted to the reagent kit
- Open Reagent Compatibility: works with most commercial magnetic bead extraction kits, with diverse mixing and magnetic absorption modes for reagent optimization
- Automatic Tip Comb Loading: mechanized comb loading and unloading with a reported success rate above 99.99%
- Kit Detection and Position Identification: detects missing kits and verifies all eight plate positions, even if a plate is bumped
- Splash and Contamination Control: drip shield, software-controlled bead drying, disposable consumables, UV sterilization, and fan exhaust
- Self-Check and Power-Off Protection: verifies system status at startup and protects runs during power interruptions
- Accessible Front Door: pause a run at any point to inspect progress or optimize reagents
- 7-Inch Touchscreen Control: edit parameters and programs without a computer, using shortcut buttons or a mouse
- 100-Program Memory with Run Reports: eight preset programs, 100 user programs, built-in SD storage, and automatic reports for traceability
- Encrypted Operating System: blocks unauthorized changes to parameters and programs
- Network Expansion Options: expandable Ethernet remote control, Wi-Fi, and 4G connectivity
- Automation Ready: optional integration with robotic arms and liquid handling systems for high-throughput workflows
Theory and Method
Magnetic bead extraction isolates nucleic acids on the surface of silica-coated paramagnetic particles. A lysis buffer with chaotropic salts breaks open cells and viral particles and strips proteins from DNA and RNA. Under these conditions, nucleic acids bind tightly to the bead surface. Ethanol-based wash buffers then remove salts, proteins, and PCR inhibitors while the nucleic acids stay attached. A low-salt elution buffer, usually heated, finally releases purified DNA or RNA into solution.
The ToronNAE™ Series uses the magnetic rod method with a center rotary table. Each plate position holds one reagent step: lysis, washes, or elution. Magnetic rods sit inside a disposable tip comb that keeps them out of the liquid. The comb oscillates in each well to mix the sample, using editable mixing and magnetic absorption patterns. When the rods drop into the comb, they collect the beads, and the table rotates the next plate into position for release.
Moving beads instead of liquids avoids pipetting losses and keeps each sample in its own column of wells. Heated lysis speeds cell disruption and enzyme digestion, while heated elution improves nucleic acid recovery from the beads. The software controls bead drying time so residual ethanol evaporates without overdrying the beads. An automatic comb loading and unloading mechanism keeps rod positioning repeatable across runs. Together these controls deliver a CV below 5% and a positive rate above 95% at 100 copies.
High-Throughput Nucleic Acid Extractor - ToronNAE™ Series Technical Specifications
Model-Specific Specifications
| Parameter | ToronNAE™ 96 | ToronNAE™ 48 | ToronNAE™ 24 |
|---|---|---|---|
| Throughput | 1 to 96 samples | 1 to 48 samples | 1 to 24 samples |
| Process Volume | 50 µL to 1000 µL | 50 µL to 3000 µL | 50 µL to 10,000 µL |
| Reaction System | 1000 µL | 3000 µL | 10,000 µL |
| Consumables | 96 × 2.2 mL deep-well plate + 96 magnetic rod comb | 48 × 5 mL deep-well plate (3 mL cartridge) + 48 magnetic rod comb | 24 × 14 mL deep-well plate (10 mL cartridge) + 24 magnetic rod comb |
Shared Specifications (All Models)
| Parameter | Specification |
|---|---|
| Working Principle | Magnetic bead method, magnetic rod type with center rotary table |
| Extraction Time | 30 to 60 min per run (kit dependent) |
| Plate Positions | 8 (2 heated as standard; customizable up to 4 heated) |
| Purification Accuracy | 100-copy sample positive rate > 95% |
| Stability | CV < 5% |
| Lysis Temperature | Room temperature to 120 °C |
| Elution Temperature | Room temperature to 120 °C |
| Programmable Temperature Range | RT + 5 °C to 120 °C |
| Mixing | User-editable mixing modes |
| Tip Comb Handling | Automatic loading and unloading (success rate > 99.99%) |
| Operation Interface | 7-inch color touchscreen, 3 shortcut buttons, mouse support |
| Built-In Programs | 8 preset programs; up to 100 programs stored |
| Program Management | New, edit, delete, save as |
| Software Security | Operating system encryption against tampering with parameters and programs |
| Expansion Interface | Expandable Ethernet remote control, Wi-Fi, 4G network |
| Data Storage | Built-in SD card |
| Reporting | Automatic run report generation |
| Safety Functions | Startup self-check, power-off protection, missing kit detection, plate position identification |
| Lighting | LED |
| Sterilization | UV light |
| Exhaust | Fan |
| Maximum Input Power | 300 W |
| Dimensions (W × D × H) | 560 × 620 × 500 mm |
| Weight | 54 kg |
Consumables
| Item | Description |
|---|---|
| ToronNAE™ 96 Magnetic Tip Comb | Disposable 96-tip comb that covers the magnetic rods |
| ToronNAE™ 96 Deep-Well Plate | 96-well deep-well plate for pre-filled extraction reagents |
| ToronNAE™ 96 Elution Plate | 96-well plate for collecting purified nucleic acid |
| ToronNAE™ 48 Magnetic Tip Comb | Disposable 48-tip comb that covers the magnetic rods |
| ToronNAE™ 48 Well Plate | 48-well plate for the 3 mL reaction system |
| ToronNAE™ 24 Magnetic Tip Comb | Disposable 24-tip comb that covers the magnetic rods |
| ToronNAE™ 24 Plate | 24 × 14.6 mL plate for the 10 mL reaction system |