Large Capacity High Speed Refrigerated Centrifuge (4 x 750 mL)
Large Capacity High Speed Refrigerated Centrifuge (4 x 750 mL) - ToronCent™ HSR LC
ToronCent HSR LC large capacity refrigerated centrifuge processes up to 3 liters per run in four 750 mL buckets while holding the chamber from -20°C to 40°C. Blood banks, cell culture facilities, and process development labs use it to harvest cells, wash media, and separate blood components in volumes that microtube instruments cannot reach. A variable frequency drive turns angle rotors as fast as 21,000 rpm for small-volume work on the same deck. The benchtop chassis measures 713 x 681 x 398 mm.
Fourteen rotor options span the range, and the choice sets the real ceiling for any given protocol. The 4 x 750 mL swing rotor turns at 4,000 rpm and 3,500 x g, which suits blood component work and cell harvesting. Angle rotors deliver the high end: the 24 x 1.5/2.2 mL rotor reaches 32,752 x g, and the 12 x 1.5/2.2 mL rotor hits the full 21,000 rpm. Maximum speed and maximum capacity therefore belong to different rotors, not to a single configuration.
Temperature control uses a variable frequency compressor under PID regulation with a dual-channel refrigeration circuit, holding 4°C within ±0.5°C even at maximum speed. Pre-cooling runs on a schedule and adapts to the rotor in place, so the chamber sits at setpoint when the first sample arrives. Aerosol-tight round and rectangular buckets contain the sample during spins of infectious or hazardous material. Rotor identification, automatic run logging, and 100 named programs round out the control package for regulated laboratories.
Large Capacity High Speed Refrigerated Centrifuge (4 x 750 mL) - ToronCent™ HSR LC Applications
The ToronCent HSR LC covers the volume range where clinical, biotechnology, and process laboratories move from tube-scale preparation to bulk separation.
- Blood Banking and Transfusion Services: separates whole blood into packed cells, plasma, and platelet fractions using the 4 x 750 mL swing rotor and dedicated blood collection tube adapters
- Clinical Diagnostics: prepares serum and plasma in batches of up to 152 vacuum collection tubes per run across four rectangular buckets
- Cell Culture and Bioprocess Development: harvests bacterial, yeast, and mammalian cultures from shake flasks and bioreactor draws without splitting the volume across multiple spins
- Molecular Biology and Nucleic Acid Preparation: pellets DNA and RNA precipitates and clears lysates on high speed angle rotors above 30,000 x g
- Protein Purification and Enzymology: collects precipitated protein and removes cell debris while the chamber holds 4°C to preserve activity
- Microbiology and Vaccine Research: concentrates bacterial cultures and viral preparations inside aerosol-tight buckets rated for biosafety handling
- Pharmaceutical Quality Control: clarifies formulation batches and supports stability programs that call for controlled-temperature separation
- High-Throughput Screening Laboratories: spins deep-well plates and standard microplates with the dedicated microplate bucket
- Environmental and Water Analysis: settles suspended solids from large-volume field samples ahead of instrumental analysis
Standards
torontech supplies the ToronCent HSR LC with the manufacturer's conformity documentation and calibration records. Supplier literature for this model does not cite specific international test standards, so laboratories operating under accredited quality programs should request the declaration of conformity at the time of order and qualify the unit under their own equipment verification procedure.
Large Capacity High Speed Refrigerated Centrifuge (4 x 750 mL) - ToronCent™ HSR LC Key Features
The design addresses the three problems that surface most often in high-volume separation work: temperature drift at speed, method repeatability across operators, and containment of hazardous samples.
- Variable Frequency Compressor with PID Control: compressor output modulates continuously instead of cycling on and off, which holds 4°C to within ±0.5°C even at maximum rotational speed
- Dual-Channel Refrigeration Circuit: two independent cooling paths keep temperature-sensitive samples at setpoint through the full acceleration and braking cycle
- Scheduled Pre-Cooling: pre-cooling parameters adjust to the installed rotor and start on a timer, so the chamber is at temperature when the analyst walks in
- AC Variable Frequency Motor: a brushless drive removes carbon dust from the chamber, eliminates brush replacement, and holds speed to better than 10 rpm
- Aerosol-Tight Buckets: sealed round and rectangular buckets contain the sample during spins of infectious or otherwise hazardous material
- 100 Named Program Groups: stored and labeled methods recall in one step, which standardizes technique across shifts and simplifies audit review
- Eleven Acceleration and Twelve Deceleration Rates: fine ramp control keeps soft pellets and density layers intact through the braking phase
- 7-Inch HD Touchscreen: setup values and live run parameters appear on one screen, and operators type target values directly rather than stepping through menus
- Automatic Run and Diagnostic Logging: the instrument records every cycle and every fault without operator action, giving QC teams a retrievable history per batch
- User-Defined Sleep Mode: the unit idles between experiments on a schedule the lab sets, which cuts energy use and reduces compressor wear
- Rotor Identification: the drive recognizes the installed rotor and limits speed to that rotor's rating before the run starts
- Short Spin Function: a momentary spin collects droplets from tube walls without programming a full cycle
- Fourteen Rotor Options: angle rotors from 48 x 1.5/2.2 mL through 6 x 100 mL, plus 4 x 500 mL and 4 x 750 mL swing rotors and a dedicated microplate bucket
- Low Acoustic Output: noise stays at or below 65 dB(A), which suits shared benches and open laboratory layouts
Theory and Method
Sedimentation under centrifugal force separates particles by size and density difference. Angular velocity sets the field strength, and rotor radius scales it, so relative centrifugal force follows RCF = 1.118 × 10⁻⁵ × r × N² with r in centimeters and N in rpm. Large-volume rotors carry heavy loads at long radius, which raises rotor stress as the square of speed and caps how fast they can safely turn. Rotor mechanics, not motor power, cap the 4 x 750 mL swing rotor at 4,000 rpm.
Swinging buckets pivot outward as the rotor spins, aligning the tube axis with the force vector. Particles travel straight down the full tube length and form a flat pellet at the bottom, which makes decanting straightforward and keeps layered separations sharp. Fixed-angle rotors hold tubes at a constant tilt, so particles strike the outer wall first and slide down to a compact pellet. Shorter path length means faster clearing at equal force, which is why the small-volume angle rotors finish a spin in less time.
Aerodynamic drag on the rotor converts mechanical energy into heat, and the load climbs steeply with speed. A fixed-output compressor overshoots at low speed and falls behind at high speed, which is where variable frequency control earns its place. The ToronCent HSR LC modulates compressor output continuously against a PID loop, so the chamber tracks setpoint through acceleration, steady state, and braking. Sample temperature stays at 4°C within ±0.5°C even during a maximum-speed run, which protects enzymes, viable cells, and labile blood fractions.
Large Capacity High Speed Refrigerated Centrifuge (4 x 750 mL) - ToronCent™ HSR LC Technical Specifications
| Parameter | Specification |
|---|---|
| Order Number | 0202253006 |
| Maximum Speed | 21,000 rpm |
| Maximum RCF | 32,752 x g |
| Maximum Capacity | 4 x 750 mL |
| Speed Accuracy | ±10 rpm |
| Time Setting Range | 1 s to 99 min 59 s; 1 min to 99 h 59 min |
| Timing Modes | Start timing, speed timing, continuous timing |
| Temperature Setting Range | -20°C to 40°C |
| Temperature Accuracy | 4°C ±0.5°C at maximum speed |
| Pre-Cooling Function | Yes, rotor-specific and schedulable |
| Refrigeration Unit | Variable frequency compressor, R134a refrigerant |
| Drive Motor | AC variable frequency, brushless |
| Display | 7-inch HD touchscreen |
| Program Storage | 100 user-defined program groups |
| Acceleration / Deceleration Rates | 11 / 12 |
| Rotor Identification | Yes |
| Short Spinning | Yes |
| Noise Level | ≤65 dB(A) |
| Voltage | AC 220 V ±22 V, 50/60 Hz (110V is also available) |
| Power Consumption | 1.6 kW |
| Dimensions (W x D x H) | 713 x 681 x 398 mm (28.1 x 26.8 x 15.7 in) |
| Packing Dimensions (W x D x H) | 860 x 810 x 620 mm (33.9 x 31.9 x 24.4 in) |
| Net Weight | 95 kg (209 lb) |
Available Rotors
Maximum speed and maximum RCF apply to each rotor individually, not to the instrument as a whole.
| Rotor | Order Number | Type | Capacity | Max Speed | Max RCF | Adapters |
|---|---|---|---|---|---|---|
| No. 1 | 0301805020 | Fixed angle | 12 x 1.5/2.2 mL | 21,000 rpm | 30,642 x g | 0.2 mL, 0.5 mL |
| No. 2 | 0301891142 | Fixed angle | 12 x 15 mL (conical) | 14,000 rpm | 21,913 x g | 5 mL conical |
| No. 3 | 0301805310 | Fixed angle | 12 x 10 mL | 15,000 rpm | 23,120 x g | 1.5 mL, 5 mL |
| No. 3A | 0301891095 | Fixed angle | 6 x 30 mL | 15,000 rpm | 23,120 x g | 5 mL, 10 mL |
| No. 4 | 0301805420 | Fixed angle | 6 x 50 mL | 13,500 rpm | 19,354 x g | 1.5, 5, 10, 15, 20, 25, 30 mL |
| No. 5 | 0301805930 | Fixed angle | 6 x 50 mL (conical) | 13,000 rpm | 17,947 x g | 15 mL conical; 50 mL round-bottom adapter included |
| No. 6 | 0301891235 | Fixed angle | 12 x 5 mL | 16,500 rpm | 19,166 x g | 0.5 mL, 1.5 mL |
| No. 7 | 0301812510 | Fixed angle | 30 x 1.5/2.2 mL | 16,000 rpm | 28,918 x g | 0.2 mL, 0.5 mL |
| No. 8 | 0301812830 | Fixed angle | 48 x 1.5/2.2 mL | 15,000 rpm | 24,663 x g | 0.2 mL, 0.5 mL |
| No. 9 | 0301891099 | Fixed angle | 6 x 100 mL | 10,000 rpm | 11,960 x g | 5, 10, 25 mL; 15 mL and 50 mL (conical/round) |
| No. 10 | 0301891236 | Fixed angle | 24 x 1.5/2.2 mL | 18,500 rpm | 32,752 x g | 0.2 mL, 0.5 mL |
| No. 11 | 0301891237 | Fixed angle | 8 x 50 mL (conical) | 11,000 rpm | 14,100 x g | 1.5, 5, 10, 15, 20, 25, 30 mL; 50 mL round-bottom adapter included |
| No. 12 | 0302850110 | Swing | 4 x 500 mL | 4,000 rpm | 3,345 x g | See bucket tables (buckets sold separately) |
| No. 13 | 0302891036 | Swing | 4 x 750 mL | 4,000 rpm | 3,500 x g | See bucket tables (buckets sold separately) |
| No. 14 | 0301891253 | Fixed angle | 8 x 50 mL | 12,000 rpm | 15,615 x g | 1.5, 5, 10, 15, 20, 25, 30 mL |
Buckets and Adapters: Rotor No. 12 (4 x 500 mL Swing)
| Bucket | Order Number |
|---|---|
| Round bucket | 0303891022 |
| Aerosol-tight round bucket | 0303891077 |
| Rectangular bucket | 0303851421 |
| Rectangular bucket with sealing cover | 0303891016 |
| Bucket Type | Adapter Configuration | Order Number |
|---|---|---|
| Round | 4 x 1 x 250 mL | 0304891142 |
| Round | 4 x 2 x 100 mL | 0304891009 |
| Round | 4 x 4 x 50 mL (conical/round) | 0304891008 |
| Round | 4 x 7 x 20 mL | 0304851485 |
| Round | 4 x 9 x 15 mL (conical/round) | 0304891083 |
| Round | 4 x 11 x 15 mL conical (cap ≤21.5 mm) | 0304891508 |
| Round | 4 x 14 x 10 mL | 0304891705 |
| Round | 4 x 19 x 5/7 mL vacuum blood collection tube | 0304851450 |
| Round | 4 x 36 x 1.5 mL | 0304891677 |
| Rectangular | 4 x 1 x 250 mL | 0304891041 |
| Rectangular | 4 x 3 x 100 mL | 0304891744 |
| Rectangular | 4 x 6 x 50 mL (conical/round) | 0304891014 |
| Rectangular | 4 x 12 x 15 mL conical | 0304891582 |
| Rectangular | 4 x 12 x 15 mL round | 0304891779 |
| Rectangular | 4 x 20 x 10 mL | 0304891077 |
| Rectangular | 4 x 31 x 5/7 mL vacuum blood collection tube | 0304891782 |
| Rectangular | 4 x 56 x 1.5 mL | 0304891603 |
Custom adapter configurations are available on request.
Buckets and Adapters: Rotor No. 13 (4 x 750 mL Swing)
| Bucket | Order Number |
|---|---|
| Round bucket | 0303891025 |
| Aerosol-tight round bucket | 0303891006 |
| Rectangular bucket | 0303891002 |
| Microplate bucket | 0303891147 |
| Bucket Type | Adapter Configuration | Order Number |
|---|---|---|
| Round | 4 x 1 x 500 mL conical | 0304891035 |
| Round | 4 x 1 x 500 mL flat | 0304891036 |
| Round | 4 x 1 x 250 mL conical | 0304891562 |
| Round | 4 x 1 x 250 mL flat | 0304891039 |
| Round | 4 x 4 x 100 mL | 0304891006 |
| Round | 4 x 5 x 50 mL (self-standing tube) | 0304891024 |
| Round | 4 x 7 x 50 mL conical | 0304891003 |
| Round | 4 x 7 x 50 mL round | 0304891004 |
| Round | 4 x 10 x 20 mL | 0304891163 |
| Round | 4 x 15 x 15 mL conical | 0304891005 |
| Round | 4 x 19 x 10 mL (self-standing tube) | 0304891732 |
| Round | 4 x 21 x 10 mL | 0304891730 |
| Round | 4 x 27 x 5/7 mL vacuum blood collection tube | 0304891728 |
| Round | 4 x 37 x 5 mL (RIA tube) | 0304891166 |
| Round | 4 x 56 x 1.5 mL | 0304891025 |
| Rectangular | 4 x 9 x 50 mL conical | 0304891015 |
| Rectangular | 4 x 9 x 50 mL round | 0304891016 |
| Rectangular | 4 x 19 x 15 mL conical | 0304891012 |
| Rectangular | 4 x 19 x 15 mL round | 0304891704 |
| Rectangular | 4 x 30 x 10 mL | 0304891709 |
| Rectangular | 4 x 38 x 5/7 mL vacuum blood collection tube | 0304891017 |
| Microplate | 4 x 2 x 96 deep-well plate | Contact torontech |
| Microplate | 4 x 4 x 96 microplate | Contact torontech |
Total stacked plate height in the microplate bucket must not exceed 67 mm.