Microvolume UV-Vis Spectrophotometer - ToronMVS™ UV Series
ToronMVS™ UV-300 and UV-500 Microvolume Spectrophotometer for DNA, RNA and Protein
The ToronMVS™ UV microvolume UV-Vis spectrophotometer measures DNA, RNA, and protein concentration and purity from just 0.5 µL to 2 µL of sample. Molecular biology labs, sequencing cores, and QC teams use it to check nucleic acid quality before PCR, NGS, or cloning. Operators pipette the sample directly onto the pedestal, lower the arm, and read results in under 6 seconds. No cuvette, dilution, or lamp warm-up is needed. The sample can be wiped away or recovered after each reading.
Two models share the same 200 nm to 800 nm optical platform. The ToronMVS™ UV-300 measures dsDNA between 2 ng/µL and 4500 ng/µL in about 5 seconds. The ToronMVS™ UV-500 extends the range to 15,000 ng/µL with an added 0.05 mm path length and adds a fluorometer mode that detects dsDNA down to 0.5 pg/µL. Both models include a cuvette mode for OD600 bacterial density measurements. A 7-inch touchscreen runs the instrument without a computer.
A pulsed xenon flash lamp and 2048-element CCD array capture the full spectrum in a single flash. The motorized arm on the UV-F lifts the upper pedestal precisely, which keeps the liquid column from breaking during measurement. Results export to a USB flash drive, and a built-in printer produces reports at the bench. The compact footprint fits easily beside a thermal cycler or biosafety cabinet.
Microvolume UV-Vis Spectrophotometer - ToronMVS™ UV Series Applications
The ToronMVS™ UV serves any lab that needs fast quantification and purity checks on small, precious samples. Its fluorometer option on the UV-F covers low-concentration samples that fall below UV absorbance limits.
- Nucleic Acid QC Before PCR and qPCR: A260 quantification and 260/280 and 260/230 purity ratios confirm template quality before amplification
- NGS Library Preparation: concentration checks on input DNA and RNA, with fluorometric quantification of dilute libraries on the UV-F
- RNA Quality Control: RNA concentration and purity measurement before reverse transcription and RNA-seq
- Định lượng protein và kháng thể: A280 measurement of purified proteins, antibodies, and peptides using known or custom extinction coefficients
- Vi sinh học và nuôi cấy tế bào: OD600 readings in cuvette mode track bacterial growth and determine induction timing
- Fluorescent Dye and Reporter Assays: GFP, fluorescein, and labeled oligonucleotide detection with the UV-F fluorescence channels
- Plasmid and Cloning Workflows: fast checks on miniprep and PCR product yields before restriction digestion or ligation
- Teaching and Core Laboratories: shared-use instruments that require no warm-up and minimal training
Microvolume UV-Vis Spectrophotometer - ToronMVS™ UV Series Key Features
The ToronMVS™ UV combines microvolume UV-Vis absorbance, OD600 cuvette mode, and optional fluorescence in a standalone benchtop unit. Each feature targets faster readings, less sample use, and more reliable QC data.
- 0.5 µL to 2 µL Sample Volume: measures precious samples without dilution, with full sample recovery after reading
- Full 200 nm to 800 nm Spectrum: captures the complete UV-Vis profile, including A230, A260, and A280, in one flash
- Phạm vi nồng độ rộng: 2 ng/µL to 4500 ng/µL dsDNA (UV-S) or up to 15,000 ng/µL (UV-F) without dilution
- Results in 5 to 6 Seconds: no lamp warm-up, so the instrument is ready to measure at startup
- Multiple Path Lengths: automatic path selection keeps concentrated samples within the linear range
- Motorized Lifting Arm (UV-F): precise pedestal motion prevents liquid column breakage and improves reading stability
- Built-In Fluorometer (UV-F): blue channel quantifies dsDNA, RNA, and protein down to 0.5 pg/µL dsDNA, with optional UV, green, or red channels
- Chế độ Cuvette OD600: dedicated LED path measures bacterial and cell culture density across 0 to 4 A
- Xenon Flash Lamp and CCD Detector: long-life pulsed source and 2048-element array give 1 nm wavelength accuracy
- 7-Inch Touchscreen, No PC Required: Android-based software runs measurements and stores results on the instrument
- USB Export and Built-In Printer: transfer data to a computer by USB flash drive or print reports directly
- Automatic Blank and Lamp Shutoff: blanks and measures when the arm closes, and switches off the light source after idle periods
- Easy-Clean Pedestal: aluminum alloy and quartz fiber surfaces wipe clean between samples
Lý thuyết và Phương pháp
UV-Vis spectrophotometry measures how much light a sample absorbs at each wavelength. The Beer-Lambert law links absorbance (A) to concentration (c) through A = εcl, where ε is the extinction coefficient and l is the path length. Nucleic acid bases absorb strongly at 260 nm, so an A260 of 1.0 at a 10 mm path equals about 50 ng/µL of dsDNA. Proteins absorb at 280 nm. Ratios such as A260/A280 and A260/A230 reveal protein, phenol, or salt contamination in a nucleic acid sample.
Microvolume measurement replaces the cuvette with surface tension. The operator places a droplet on the lower pedestal, and the arm brings a second optical fiber down to touch it. The droplet stretches into a liquid column between the two fiber ends. The instrument then sets very short path lengths of 1.0 mm, 0.2 mm, or 0.05 mm, so concentrated samples stay within the linear absorbance range. The software normalizes every reading to a 10 mm equivalent, which removes the need for dilution.
Fluorometric quantification on the UV-F takes over below the UV absorbance limit. Fluorescent dyes such as dsDNA-selective intercalators emit strongly only when bound to their target molecule. An LED excites the dye at a set wavelength, and a photodiode measures the emitted light. Signal intensity scales with target concentration across five orders of magnitude, so the instrument quantifies dsDNA down to 0.5 pg/µL. The dye's selectivity also ignores free nucleotides and RNA that would inflate an A260 reading.
Microvolume UV-Vis Spectrophotometer - ToronMVS™ UV Series Technical Specifications
Chế độ hấp thụ thể tích nhỏ
| Tham số | ToronMVS™ UV-300 | ToronMVS™ UV-500 |
|---|---|---|
| Dải bước sóng | 200 nm đến 800 nm | 200 nm đến 800 nm |
| Khối lượng mẫu | 0.5 µL đến 2.0 µL | 0.5 µL đến 2.0 µL |
| Chiều dài đường | 0.2 mm, 1.0 mm | 0.05 mm, 0.2 mm, 1.0 mm |
| Nguồn sáng | Đèn flash Xenon | Đèn flash Xenon |
| Máy dò | 2048-element linear CCD array | 2048-element linear CCD array |
| Độ chính xác bước sóng | 1 nm | 1 nm |
| Độ phân giải quang phổ | ≤ 3nm | ≤ 3nm |
| Độ chính xác hấp thụ | 0.003 Áp suất | 0.003 Áp suất |
| Độ chính xác của phép đo độ hấp thụ | 1% (7.332 Abs at 260 nm) | 1% (7.332 Abs at 260 nm) |
| Phạm vi hấp thụ | 0.04 A đến 90 A | 0.04 A đến 300 A |
| Phạm vi phát hiện axit nucleic | 2 ng/µL đến 4500 ng/µL (dsDNA) | 2 ng/µL đến 15,000 ng/µL (dsDNA) |
| Phạm vi protein A280 (BSA) | 0.1 mg/mL đến 135 mg/mL | 0.1 mg/mL đến 450 mg/mL |
| Thời gian đo | <5 giây | <6 giây |
| Pedestal Lifting | Manual arm | Motorized lifting structure |
Chế độ Cuvette (OD600)
| Tham số | ToronMVS™ UV-300 | ToronMVS™ UV-500 |
|---|---|---|
| Nguồn sáng | LED | LED |
| Bước sóng | Bước sóng 600 ± 8 | Bước sóng 600 ± 8 |
| Phạm vi hấp thụ | 0 A đến 4 A | 0 A đến 4 A |
Fluorometer Mode (ToronMVS™ UV-500 Only)
| Tham số | Đặc điểm kỹ thuật |
|---|---|
| Nguồn sáng | LED |
| Máy dò | Điốt quang |
| Dải động | 5 bậc của cường độ |
| Tuyến tính | R² ≥ 0.995 |
| Độ lặp lại | ≤ 1.5% |
| Tính ổn định | ≤ 1.5% |
| Độ nhạy | 0.5 pg/µL (dsDNA) |
| Tốc độ đo | 3 s per reading |
Fluorescence Channels (ToronMVS™ UV-500)
| Kênh | Kích thích | Khí thải | Các ứng dụng tiêu biểu |
|---|---|---|---|
| Blue (standard) | Bước sóng 460 ± 20 | 525 nm đến 570 nm | dsDNA, ssDNA, protein quantification, GFP, fluorescein |
| UV (tùy chọn) | Bước sóng 365 ± 20 | 420 nm đến 480 nm | Nucleic acid quantification, GUS reporter assays, apoptosis assays |
| Green (optional) | Bước sóng 525 ± 20 | 575 nm đến 640 nm | Rhodamine, Cy3-labeled probes, RFP, cytotoxicity assays |
| Màu đỏ (tùy chọn) | Bước sóng 625 ± 20 | 670 nm đến 725 nm | Cy5-labeled probes, RNA quantification |
Thông số kỹ thuật chung
| Tham số | ToronMVS™ UV-300 | ToronMVS™ UV-500 |
|---|---|---|
| Giao diện | Màn hình cảm ứng điện dung 7 inch | Màn hình cảm ứng điện dung 7 inch |
| Phần mềm | Dựa trên Android | Dựa trên Android |
| Xuất dữ liệu | Ổ đĩa flash USB | Ổ đĩa flash USB |
| Máy in | Built-in | Built-in |
| Vật liệu bệ đỡ | Hợp kim nhôm và sợi thạch anh | Hợp kim nhôm và sợi thạch anh |
| Điện áp hoạt động | DC 24 V, 2 A | DC 24 V, 2 A |
| Sức mạnh hoạt động | 25 W | 25 W |
| Công suất dự phòng | 5 W | 5 W |
| Kích thước (W × D × H) | 210 × 265 × 181 mm | 208 × 320 × 186 mm |
| Trọng lượng máy | 2.8 kg | 3.6 kg |
Phụ kiện
| Mục | Mô tả Chi tiết |
|---|---|
| Cuvette OD600 | Cuvette for bacterial and cell culture density measurement |
| Optional Fluorescence Channel | UV, green, or red channel configuration for the ToronMVS™ UV-500 |