Integrated Nucleic Acid Extraction and PCR System
Integrated Nucleic Acid Extraction and PCR System - ToronPCR™ EG
ToronPCR™ EG Series Automated Nucleic Acid Extraction and Real-Time PCR System
The ToronPCR™ EG integrated nucleic acid extraction and PCR system takes a raw sample through extraction, amplification, and fluorescence detection inside one sealed cartridge. Clinical labs, public health teams, food safety inspectors, and veterinary clinics use it when they need molecular results close to the point of sampling. The operator adds the sample and magnetic beads, inserts the cartridge, and selects a program. Hands-on time stays at 2 minutes or less per sample. The instrument handles every remaining step without manual pipetting.
The series comes in three sizes: the ToronPCR™ EG1, EG2, and EG4 process one, two, and four samples at a time. Each sample position runs independently, so one channel can start while another is mid-cycle. Labs can combine units and control up to 16 sample channels together. This modular layout lets a site add capacity as testing demand grows instead of buying a large batch instrument upfront. The compact footprint fits on a standard bench or inside a mobile lab.
Every cartridge arrives with freeze-dried extraction and PCR reagents already loaded at the factory. Four fluorescence channels let one sample screen for several targets in a single run. Operators choose qualitative, quantitative, or SNP genotyping output depending on the assay. The 10.5-inch touchscreen and Windows-based software guide each run, while LIS and cloud database connections are available as expansion options. A built-in self-check routine verifies system status to support reliable results.
Integrated Nucleic Acid Extraction and PCR System - ToronPCR™ EG Applications
The ToronPCR™ EG brings sample to answer PCR testing to sites that lack a dedicated molecular lab or need results faster than a central lab can deliver them. Its closed cartridge design suits decentralized testing across clinical, public health, and industrial settings.
- Bệnh viện và Phòng thí nghiệm lâm sàng: rapid detection of infectious disease pathogens in patient samples, supporting faster triage decisions (subject to assay and regulatory clearance)
- Phòng thí nghiệm y tế công cộng: outbreak response and surveillance testing for respiratory, enteric, and vector-borne pathogens
- Point-of-Entry and Customs Screening: on-site nucleic acid testing of travelers, cargo, and imported goods at borders, airports, and seaports
- Kiểm tra an toàn thực phẩm: detection of pathogens such as Salmonella, Listeria, and E. coli, plus GMO screening and species identification in food products
- Nhận dạng pháp y: DNA-based screening and species identification in forensic and law enforcement laboratories
- Nghiên cứu ung thư: quantitative and SNP assays for tumor-associated gene mutations and expression markers
- Prenatal and Genetic Testing Research: genotyping of target variants with SNP assay workflows
- Veterinary and Pet Hospitals: in-clinic PCR testing for companion animal infections such as canine parvovirus and feline respiratory pathogens
- Mobile and Field Laboratories: compact molecular testing in vehicles, temporary clinics, and remote field sites
Integrated Nucleic Acid Extraction and PCR System - ToronPCR™ EG Key Features
The ToronPCR™ EG combines extraction, amplification, and detection in a compact format built for decentralized molecular testing. Each feature targets faster setup, lower contamination risk, and flexible capacity.
- Sample to Answer Workflow: hands-on time of 2 minutes or less per sample, limited to adding the sample and magnetic beads, while the instrument completes every downstream step
- Pre-Loaded Freeze-Dried Reagents: extraction and PCR reagents come sealed in each cartridge, removing manual reagent preparation and the pipetting errors that come with it
- Closed Single-Use Cartridge: one dedicated cartridge per sample with a tight seal that contains aerosols and prevents cross-contamination between samples
- Four-Channel Fluorescence Detection: optical bands matched to FAM, HEX, ROX, and CY5 dyes support multiplex assays with several targets per sample
- Qualitative, Quantitative, and SNP Modes: switch between positive or negative calls, copy-number quantitation from a built-in standard curve, and genotyping as the assay requires
- Modular, Scalable Throughput: choose 1-, 2-, or 4-sample units and combine them to control up to 16 sample channels, with each channel running independently
- Open Assay Compatibility: open software works with commercially available magnetic bead extraction and detection kits, so labs avoid lock-in to a single reagent supplier
- Chu trình nhiệt nhanh: heating rates up to 10 °C/s and cooling rates up to 8 °C/s shorten total amplification time
- Per-Channel UV Sterilization: an independent UV lamp in each sample channel supports routine decontamination between runs
- Built-In Self-Check: automatic system verification helps confirm the instrument is ready and each result is valid
- Role-Based User Management: administrator-controlled access levels protect methods and data from unauthorized changes
- 10.5-Inch Touchscreen with Windows Software: a visual interface simplifies program selection, and the software supports custom function configuration
- Data and Connectivity Options: RS232, USB, and LAN ports, barcode scanner input for sample and reagent data, printer output for test reports, and expandable LIS and cloud database links.
Lý thuyết và Phương pháp
The ToronPCR™ EG extracts nucleic acids through magnetic bead solid-phase separation. A lysis buffer breaks open cells and viral particles, and chaotropic salts strip proteins away from DNA and RNA. Under these conditions, nucleic acids bind to silica-coated paramagnetic beads. A magnet below the cartridge pulls the beads down while the system moves liquid away from them. Three wash cycles remove salts and inhibitors, and heated elution buffer then releases purified nucleic acid from the bead surface.
A rotating piston valve at the cartridge base controls all liquid transfer. Each valve position connects the piston chamber to a different well, so the system draws and pushes fluid through the sample, wash, and elution chambers in sequence. The eluate then moves into the PCR reaction area and rehydrates the freeze-dried amplification reagents. The cartridge stays sealed for the entire run. This closed path keeps amplified product inside the consumable and limits carryover between samples.
Real-time PCR amplifies target sequences through repeated heating and cooling cycles while fluorescent probes report product formation. The thermal module ramps at up to 10 °C/s, which shortens each cycle. Four optical channels read FAM, HEX, ROX, and CY5 dyes, so one reaction can carry up to four probe targets, including an internal control. The cycle threshold (Ct) at which fluorescence crosses baseline correlates with starting template quantity. The software converts Ct values into copy numbers against a built-in standard curve.
Integrated Nucleic Acid Extraction and PCR System - ToronPCR™ EG Technical Specifications
Thông số kỹ thuật cụ thể của từng mẫu xe
| Tham số | ToronPCR™ EG1 | ToronPCR™ EG2 | ToronPCR™ EG4 |
|---|---|---|---|
| Thông lượng | 1 mẫu | 2 mẫu | 4 mẫu |
| Kích thước (W × D × H) | 124 × 292 × 427 mm | 248 × 292 × 427 mm | 496 × 292 × 427 mm |
| Trọng lượng máy | Không được nêu rõ trong nguồn. | Không được nêu rõ trong nguồn. | Không được nêu rõ trong nguồn. |
Thông số kỹ thuật chung (Tất cả các mẫu)
| Tham số | Đặc điểm kỹ thuật |
|---|---|
| Maximum Combined Capacity | Up to 16 sample channels controlled together |
| Consumable Configuration | 4 reagent chambers (pre-packaged liquid reagents); 1 sample chamber with sealing cover; 1 detection well for PCR amplification and fluorescence detection |
| Sample Chamber Capacity | 3 mL maximum; approx. 2.5 mL working volume |
| Magnetic Separation Method | Bottom magnetic absorption |
| Extraction Heating | Lysis well and elution well heated |
| Extraction Heating Range | Nhiệt độ phòng đến 120 °C |
| Extraction Temperature Accuracy | ± 0.5 ° C |
| Thời gian khai thác | 25 to 30 min per sample (typical, depends on reagent protocol) |
| Extraction Consistency | CV ≤ 5% |
| PCR Average Heating Rate (50 °C to 95 °C) | ≥ 6 °C/s |
| PCR Maximum Heating Rate (50 °C to 95 °C) | ≥ 10 °C/s |
| PCR Average Cooling Rate (95 °C to 50 °C) | ≥ 4.5 °C/s |
| PCR Maximum Cooling Rate (95 °C to 50 °C) | ≥ 8 °C/s |
| PCR Temperature Accuracy | ≤ ±0.2 °C |
| Độ chính xác của việc kiểm soát nhiệt độ mô-đun | ≤ ±0.2 °C (at 55 °C) |
| Fluorescence Detection Channels | 4 (470-510 nm, 530-565 nm, 585-620 nm, 630-665 nm) |
| Compatible Fluorescent Dyes | FAM, HEX, ROX, CY5 |
| Thời gian phát hiện | 35 to 50 min per sample (typical, depends on reagent protocol) |
| PCR Detection Types | Qualitative, quantitative, SNP |
| Manual Hands-On Time | ≤ 2 min per sample |
| Giao diện | Màn hình cảm ứng 10.5-inch |
| Khử trùng | Independent UV sterilization lamp per sample channel |
| Phần mềm | Windows-based, supports custom function configuration |
| Quản lý người dùng | Administrator-level authorization control |
| Đầu ra báo cáo | External printer connection for test reports |
| Giao diện bên ngoài | 1 × RS232, 1 × USB-B, 1 × LAN |
| Quản lý thông tin | External barcode scanner for sample and reagent data; expandable LIS connection; expandable cloud database connection |
Hàng tiêu dùng
| Mục | Mô tả Chi tiết |
|---|---|
| ToronPCR™ EG Single-Use Cartridge | Disposable integrated cartridge with pre-loaded freeze-dried extraction and PCR reagents, sample chamber, three wash chambers, elution chamber, and PCR reaction area |