Full-Spectrum Microvolume Spectrophotometer
Full-Spectrum Microvolume Spectrophotometer - ToronMVS™ FS
ToronMVS™ FS Full-Spectrum Microvolume Spectrophotometer with Cuvette Capability
The ToronMVS™ FS full-spectrum microvolume spectrophotometer measures absorbance across 190 nm to 1100 nm using as little as 0.5 µL of sample. Genomics cores, protein labs, and biotech QC teams use it to quantify nucleic acids, proteins, and labeled molecules without dilution. Six path lengths, as short as 0.02 mm, extend the dsDNA range up to 30,000 ng/µL. Each reading finishes in 8 seconds or less. A slit cuvette mode handles OD600 culture measurements, and a built-in fluorometer quantifies samples below 2 ng/µL.
The instrument runs on a 10-inch touchscreen that tilts to suit each operator's working position. No external computer is needed, which saves bench space and simplifies validation. A built-in camera confirms that the liquid column has formed correctly before each reading. The software raises an alarm when purity ratios fall below user-set limits. Optional face recognition login restricts access to authorized users and protects stored data.
Results export by USB flash drive, print on the built-in printer, or transfer in real time over a local network by FTP. Built-in protein, dye, and standard curve libraries store recurring methods so operators can recall them instead of re-entering settings. Integrated verification and calibration functions work with an optional performance verification solution. The pedestal design allows sample loading with either hand. No warm-up is required, so the instrument measures as soon as it powers on.
Full-Spectrum Microvolume Spectrophotometer - ToronMVS™ FS Applications
The ToronMVS™ FS serves labs that need full UV-Vis-NIR spectral data, very high concentration range, and fluorescence sensitivity in one benchtop instrument. Its preset methods cover nucleic acids, proteins, colorimetric assays, and cell culture density.
- Định lượng axit nucleic: dsDNA, ssDNA, RNA, oligonucleotide, and primer quantification with A260, A280, and A230 values and purity ratios
- High-Concentration Samples: direct measurement of plasmid preps and genomic DNA up to 30,000 ng/µL without dilution
- Labeled Probe Analysis: concentration and labeling checks on dye-labeled primers and probes using full-spectrum scans
- Protein and Antibody Quantification: A280 measurement of proteins, antibodies, and peptides with custom extinction coefficients
- Colorimetric Protein Assays: preset BCA, Bradford, Lowry, and 660 nm protein assay methods
- Microbiology and Cell Culture: OD600 bacterial density measurement in slit cuvettes for growth curves and induction timing
- Định lượng huỳnh quang: low-concentration dsDNA, RNA, protein, and oligo measurement, plus fluorescence kinetics
- Nanoparticle and Dye Characterization: absorption peak identification across the 190 nm to 1100 nm range
Full-Spectrum Microvolume Spectrophotometer - ToronMVS™ FS Key Features
The ToronMVS™ FS combines full-spectrum microvolume absorbance, cuvette OD600, and fluorescence in a standalone instrument. Each feature targets wider measurement range, reliable readings, and secure data handling.
Precise Verification
Broad-Range Spectral Detection
Nhận diện khuôn mặt
Sample Monitoring
Lưu trữ ứng dụng
Fluorometer Mode
Dễ dàng hoạt động
Cuvette Function
- 190 nm to 1100 nm Spectral Range: full UV-Vis-NIR coverage with automatic path switching or fixed-wavelength readings
- Sample Volume of 0.5 µL to 2 µL: conserves precious samples, with recovery after measurement
- dsDNA Range up to 30,000 ng/µL: six path lengths down to 0.02 mm measure concentrated samples without dilution
- Results in 8 Seconds or Less: no warm-up, so measurements start immediately at power-on
- Built-In Camera Monitoring: confirms liquid column formation before each reading to prevent unstable results
- Purity Ratio Alarm: flags samples whose purity ratios fall below set limits
- Fluorometer Mode: blue channel as standard, optional red channel, with detection down to 0.5 pg/µL dsDNA and real-time kinetics
- OD600 Slit Cuvette Mode: measures bacterial density with low sample consumption across 0 to 4 Abs
- 10-Inch Adjustable-Angle Touchscreen: tilts to the operator's preferred viewing angle and runs without a PC
- Optional Face Recognition Login: controls user access at the source and protects data collection, storage, and retrieval
- Xuất dữ liệu linh hoạt: USB flash drive, built-in printer, and real-time FTP transfer to networked computers
- Built-In Method Libraries: stored protein, dye, and standard curve presets remove repeated setup
- Verification and Calibration Functions: on-board routines with an optional performance verification solution
- Two-Handed Sample Loading: pedestal layout allows comfortable loading with either hand
Lý thuyết và Phương pháp
UV-Vis spectrophotometry measures how much light a sample absorbs at each wavelength. The Beer-Lambert law links absorbance (A) to concentration (c) through A = εcl, where ε is the extinction coefficient and l is the path length. Nucleic acids absorb strongly at 260 nm, proteins at 280 nm, and many contaminants near 230 nm. Ratios such as A260/A280 and A260/A230 therefore reveal protein, phenol, or salt carryover. Extending the range to 1100 nm adds near-infrared data for dyes, nanoparticles, and turbidity checks.
Microvolume measurement replaces the cuvette with surface tension. The operator places a droplet on the lower pedestal, and the arm lowers a second optical fiber onto it. The droplet stretches into a liquid column between the two fiber ends. The ToronMVS™ FS then reads the column at path lengths as short as 0.02 mm, so even very concentrated samples stay within the detector's linear range. Software normalizes each reading to a 10 mm equivalent, giving an absorbance range up to 600 A.
A 2048-pixel linear CMOS array captures the full spectrum in one exposure, with 1 nm wavelength accuracy and resolution of 1.6 nm or better. For OD600, light passes through a slit cuvette, and the reading reflects light scattered by cells rather than true absorption. The fluorometer mode measures light emitted by target-specific dyes instead of absorbed light. Signal scales with target concentration, which pushes dsDNA detection down to 0.5 pg/µL.
Full-Spectrum Microvolume Spectrophotometer - ToronMVS™ FS Technical Specifications
Microvolume Absorbance Mode
| Tham số | Đặc điểm kỹ thuật |
|---|---|
| Dải bước sóng | 190 nm đến 1100 nm |
| Khối lượng mẫu | 0.5 µL đến 2 µL |
| Optical Path Lengths | 0.02 mm, 0.03 mm, 0.05 mm, 0.1 mm, 0.2 mm, 1 mm |
| Nguồn sáng | Không được nêu rõ trong nguồn. |
| Máy dò | 2048-pixel linear silicon CMOS array |
| Độ chính xác bước sóng | 1 nm |
| Độ phân giải bước sóng | ≤ 1.6 nm (FWHM at Hg 253.652 nm) |
| Độ chính xác hấp thụ | 0.003 Abs (1 mm path) |
| Độ chính xác của phép đo độ hấp thụ | ±1% (7.332 Abs at 260 nm) |
| Phạm vi hấp thụ | 0.04 A to 600 A (at 260 nm, 10 mm equivalent) |
| Phạm vi phát hiện axit nucleic | 2 ng/µL to 30,000 ng/µL (dsDNA) |
| A280 Protein Range (BSA) | 0.1 mg/mL đến 900 mg/mL |
| Thời gian thử nghiệm | ≤ 8 giây |
| Vật liệu bệ đỡ | Aluminum alloy and quartz fiber |
OD600 Cuvette Mode
| Tham số | Đặc điểm kỹ thuật |
|---|---|
| Loại cuvet | Slit cuvette |
| Phạm vi hấp thụ | 0 Abs to 4.0 Abs |
| Độ ổn định hấp thụ | 0 to 3 Abs: ≤ 0.5%; 3 to 4 Abs: ≤ 2% |
| Độ lặp lại của độ hấp thụ | 0 to 3 Abs: ≤ 0.5%; 3 to 4 Abs: ≤ 2% |
| Độ chính xác của phép đo độ hấp thụ | 0 to 2 Abs: ≤ 0.005 Abs; 2 to 3 Abs: ≤ 1%; 3 to 4 Abs: ≤ 2% |
Fluorometer Mode
| Tham số | Đặc điểm kỹ thuật |
|---|---|
| Tuyến tính | R² > 0.995 |
| Độ lặp lại | <1.5% |
| Tính ổn định | <1.5% |
| Độ nhạy | 0.5 pg/µL (dsDNA) |
| Blue Channel (Standard) | Excitation 460 nm ± 20 nm; emission 525 nm to 570 nm |
| Red Channel (Optional) | Excitation 625 nm ± 20 nm; emission 670 nm to 725 nm |
Thông số kỹ thuật chung
| Tham số | Đặc điểm kỹ thuật |
|---|---|
| Giao diện | 10-inch adjustable-angle touchscreen |
| Phần mềm | Dựa trên Android |
| Sample Monitoring | Built-in camera for liquid column verification |
| Xuất dữ liệu | USB flash drive, built-in printer, FTP over local network |
| Người dùng truy cập | Optional face recognition module |
| Điện áp đầu vào | DC 24 V, 2 A |
| Power | 25 W |
| Kích thước (W × D × H) | 260 × 333 × 346 mm |
| Trọng lượng máy | 5.8 kg |
Phụ kiện tùy chọn
| Mục | Mô tả Chi tiết |
|---|---|
| Performance Verification Solution | Reference solution for on-board instrument verification and calibration |
| Mô-đun nhận dạng khuôn mặt | Biometric login for controlled user access |
| Red Fluorescence Channel | Additional channel for Cy5-labeled probes and RNA quantification dyes |
| OD600 Cuvette | Slit cuvette for bacterial culture density measurement |