Single-Mode Microplate Reader - ToronMPR™ S
ToronMPR™ S Single-Mode Absorbance Microplate Reader for ELISA Workflows
The ToronMPR™ S single-mode microplate reader measures absorbance across 96-well plates for ELISA, colorimetric, and enzyme kinetic assays. Clinical labs, research groups, and food and veterinary testing facilities use it as a dedicated, easy-to-run absorbance reader. An 8-position filter wheel covers 340 nm to 750 nm and ships with the four most common ELISA wavelengths installed. The reader scans a full plate in about 6 seconds in fast mode. Results appear on a 7-inch touchscreen with no PC required.
The instrument runs as a standalone unit or connects to a computer for real-time data transfer. Built-in control and analysis software handles plate layouts, standard curves, and result reports directly on the touchscreen. Operators can also work with an external mouse. Internal memory stores up to 1,000 measurement programs and results, and data exports by USB flash drive. An optional printer produces reports at the bench.
A built-in shaker mixes the plate before reading, with adjustable time and speed. Absorbance accuracy reaches ±0.005 Abs across 0 to 2 Abs, with repeatability CV of 0.3% or less up to 3 Abs. The halogen light source and photodiode detector deliver a 0 to 4.0 Abs measurement range at 0.001 Abs resolution. The compact 295 × 440 × 225 mm footprint suits crowded benches. A universal 100 V to 240 V power input runs on standard North American outlets.
Single-Mode Microplate Reader - ToronMPR™ S Applications
The ToronMPR™ S fits any lab that reads colorimetric endpoints in 96-well plates. Its filter wheel accepts a broad library of optional wavelengths for assays beyond ELISA.
- Clinical ELISA Testing: endpoint reading of infectious disease, hormone, and tumor marker immunoassays at 450 nm with 630 nm reference (subject to assay and regulatory clearance)
- Research Immunoassays: antibody titer, cytokine, and antigen quantification using sandwich, competitive, and indirect ELISA formats
- Khả năng sống và sự phát triển của tế bào: MTT, CCK-8, and similar colorimetric assays read between 450 nm and 570 nm
- Định lượng protein: BCA, Bradford, and Lowry assays with the matching optional filters
- Xét nghiệm hoạt tính enzyme: endpoint and kinetic measurement of chromogenic substrates such as p-nitrophenyl phosphate at 405 nm
- Kiểm tra an toàn thực phẩm: ELISA kits for mycotoxins, antibiotic residues, allergens, and pesticide residues
- Thú y và Thú y: herd screening ELISAs for livestock and poultry diseases
- Bacterial Growth Monitoring: OD readings of microbial cultures in 96-well plates using 600 nm or 620 nm filters
- Phòng thí nghiệm giảng dạy: straightforward absorbance training with touchscreen operation and no PC setup
Single-Mode Microplate Reader - ToronMPR™ S Key Features
Giao diện phần mềm
Giao diện hoạt động
The ToronMPR™ S combines a filter-based optical system, standalone software, and plate shaking in a compact benchtop reader. Each feature targets reliable ELISA results with minimal setup.
- 6-Second Full-Plate Reads: fast mode scans 96 wells in about 6 seconds, with standard single- and dual-wavelength modes for routine work
- 8-Position Filter Wheel: 405 nm, 450 nm, 492 nm, and 630 nm filters installed, with open positions for optional wavelengths
- Wide Filter Library: more than 25 optional filters between 340 nm and 750 nm cover protein, cell viability, and enzyme assays
- Dual-Wavelength Correction: reference wavelength subtraction removes optical artifacts from plates and condensation
- High Photometric Accuracy: ±0.005 Abs accuracy across 0 to 2 Abs and repeatability CV of 0.3% or less up to 3 Abs
- Built-In Plate Shaker: mixes reagents before reading with adjustable time and speed
- Màn hình cảm ứng điện dung 7 inch: visual plate layout and touch input remove the need for a keyboard or PC
- Standalone or PC-Connected Operation: run independently or stream results to a computer in real time
- Included Control and Analysis Software: handles plate setup, standard curves, and reporting on the instrument or PC
- Bộ nhớ 1,000 chương trình: stores measurement programs and results internally, with USB flash drive export
- Ba cổng USB: connect a computer, printer, and USB flash drive at the same time
- Đầu vào nguồn điện phổ thông: 100 V to 240 V AC at 50 Hz or 60 Hz for use in any region
Lý thuyết và Phương pháp
Absorbance microplate reading applies the Beer-Lambert law to each well of a plate. A halogen lamp sends broadband light through an interference filter, which passes a narrow band around the chosen wavelength. The beam travels vertically through the sample in each well. A photodiode below the plate measures how much light gets through. The reader compares that signal with a reference reading and reports optical density, which scales with the concentration of the colored product.
ELISA links that optical density to the amount of a target molecule. An antibody captures the target in the well, and an enzyme-labeled detection antibody binds to it. The enzyme, often horseradish peroxidase, converts a colorless substrate such as TMB into a colored product. A stop solution turns the product yellow and fixes the color for reading at 450 nm. More target produces more enzyme activity and a deeper color, so the reader can calculate concentration from a standard curve.
Dual-wavelength reading improves accuracy. The reader measures each well at the primary wavelength and again at a reference wavelength, such as 630 nm, where the colored product barely absorbs. Subtracting the reference value removes errors from scratches, fingerprints, condensation, and uneven plastic. The built-in shaker mixes reagents before the read so the color is uniform throughout each well. Fast mode reads all 96 wells in about 6 seconds, while common mode trades speed for averaging.
Single-Mode Microplate Reader - ToronMPR™ S Technical Specifications
| Tham số | Đặc điểm kỹ thuật |
|---|---|
| Chế độ phát hiện | Hấp thụ |
| Định dạng tấm | đĩa vi giếng 96 giếng |
| Nguồn sáng | Halogen lamp, 6 V, 10 W |
| Dải bước sóng | 340 nm đến 750 nm |
| Lựa chọn bước sóng | 8-position filter wheel |
| Bộ lọc tiêu chuẩn | 405nm, 450nm, 492nm, 630nm |
| Độ chính xác bước sóng | ≤ ±2 nm |
| Máy dò | Điốt quang |
| Độ nhạy của máy dò | ≥ 0.01 A |
| Phạm vi hấp thụ | Từ 0 Abs đến 4.000 Abs |
| Độ phân giải | 0.001 Áp suất |
| Tuyến tính | R² ≥ 0.995 (0 Abs to 3.0 Abs) |
| Độ chính xác của phép đo độ hấp thụ | 0 to 2 Abs: ≤ ±0.005 Abs; 2 to 3 Abs: ≤ ±0.01 Abs; 3 to 4 Abs: ≤ ±1.5% |
| Absorbance Repeatability (CV) | Từ 0 đến 3 kháng thể: ≤ 0.3%; Từ 3 đến 4 kháng thể: ≤ 1% |
| Độ ổn định hấp thụ | 0 to 3 Abs: ≤ 0.005 Abs; 3 to 4 Abs: ≤ 2.0% |
| Read Time (96 Wells) | Fast mode: 6 s; single wavelength: < 15 s; dual wavelength: < 28 s |
| Lắc đĩa | Built-in, adjustable time and speed |
| Plate Incubation | Không bao gồm |
| Giao diện | 7-inch high-resolution capacitive color touchscreen |
| Đầu vào | Touchscreen, external mouse |
| Phần mềm | Built-in control and data analysis software; PC software included |
| Lưu trữ dữ liệu | Up to 1,000 measurement programs and results |
| Xuất dữ liệu | USB flash drive, real-time PC transfer |
| cổng | 3 × USB (computer, printer, USB flash drive) |
| điện áp | AC 100 V to 240 V, 50/60 Hz, 2 A |
| Kích thước (W × D × H) | 295 × 440 × 225 mm |
| Trọng lượng máy | 10 kg |
Bộ lọc tùy chọn
| Wavelength Group | Bộ lọc có sẵn |
|---|---|
| UV and Violet | 340 nm, 360 nm, 380 nm, 405 nm (standard), 415 nm |
| Màu xanh da trời | 450 nm (standard), 470 nm, 490 nm, 492 nm (standard) |
| màu xanh lá | 510 nm, 520 nm, 532 nm, 540 nm, 546 nm, 560 nm, 562 nm, 570 nm, 578 nm |
| Vàng và Cam | 590nm, 595nm, 600nm, 620nm |
| đỏ | 630 nm (standard), 646 nm, 650 nm, 663 nm, 690 nm, 700 nm, 750 nm |
Phụ kiện
| Mục | Mô tả Chi tiết |
|---|---|
| Replacement Halogen Lamp | 6 V, 10 W light source |
| Máy in | Bench printer for measurement reports |
| Giấy in | Paper rolls for the optional printer |
| Absorbance Validation Plate | Optical performance plate for routine accuracy checks |
| Phần mềm phân tích | PC control and data analysis software |